Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells
Joint Authors
Li, Jing
Setiawan, Melina
Wu, Hong
Beuerman, Roger W.
Zhao, Peiquan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Previous studies showed marked decrease of multiple Toll-like receptor (TLR) expression in corneal and conjunctival epithelial cells upon culture in vitro.
The aim of this study was to identify factor(s) which regulate TLR expression.
Primary human conjunctival epithelial cells and immortal conjunctival (IOBA-NHC) and corneal epithelial cell lines (HCET) were used.
The effect of various cytokines, hypoxia, mechanical wounding, and airlifting culture on TLR expression was examined by quantitative PCR and western blot analysis.
Ligand stimulated TLR activation was analyzed.
TLR mRNA expression increased modestly when cultured monolayered cells were stimulated by TNF-α, IL-1α, IL-1β, IL-6, IL-8, IFN-γ (about 2-fold), hypoxia (2.1- to 4.8-fold selectively), and wounding (3.1- to 9.3-fold).
In airlifted multilayered cells, TLR expression increased 7.8- to 25.9-fold compared to monolayered cells.
Airlifted cells showed increased response to low concentrations of lipopolysaccharide (LPS) and peptidoglycan (PGN) stimulation.
NFκB inhibition prevented the formation of cell sheets and led to the collapse of already-formed multilayered structure and the simultaneous reduction of TLR mRNA level.
In conclusion, our study showed that the conjunctival epithelial cell expressed TLR was sensitive to various stimulants, and a multilayered epithelium-like structure was needed to maintain TLR expression.
American Psychological Association (APA)
Li, Jing& Setiawan, Melina& Wu, Hong& Beuerman, Roger W.& Zhao, Peiquan. 2014. Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043605
Modern Language Association (MLA)
Li, Jing…[et al.]. Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043605
American Medical Association (AMA)
Li, Jing& Setiawan, Melina& Wu, Hong& Beuerman, Roger W.& Zhao, Peiquan. Regulation of Toll-Like Receptor Expression in Human Conjunctival Epithelial Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043605