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The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model
Joint Authors
Torres-Poveda, K.
Burguete-García, A. I.
Alcocer González, Juan Manuel
García Paz, Flor
Morales Ortega, Ausencio
Santander González, Abimelec
Moreno, José
Hernandez Marquez, Eva
Bermúdez Morales, Victor
Madrid-Marina, V.
Peralta-Zaragoza, Oscar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
The goal of the present study was to investigate the effect of IL-12 expressed in plasmid on the Th1 cytokine profile in an experimental HPV16-positive murine tumor model and the association with the IL-12’s antitumor effect.
Methods.
Mice were injected with BMK-16/myc cells to establish HPV16-positive tumor and then pNGVL3-mIL-12 plasmid; pcDNA3 plasmid or PBS was injected directly into tumor site.
The antitumor effect of the treatment was evaluated and the cytokines expression profile in each tumor tissue was analyzed.
Results.
Treatment with pNGVL3-mIL-12 plasmid had a significant antitumor effect, and a Th2-Th3-type cytokines prolife was detected in the murine tumor model with expression of the cytokines IL-10, IL-4, and TGF-β1.
However, after the tumor was treated with three intratumoral injections of plasmid containing IL-12 cDNA, it showed a cytokine profile associated with Th1 with expression of IL-2, IL-12, and IFN-γ cytokines and reduced expression of IL-10, IL-4, and TGF-β1.
Conclusions.
The treatment with the IL-12 gene in the experimental HPV16-positive tumor model promoted the activation of the cellular immune response via expression of a Th1-type cytokine profile and was associated with the inhibition of tumor growth.
Thus, IL-12 treatment represents a novel approach for gene therapy against cervical cancer.
American Psychological Association (APA)
García Paz, Flor& Madrid-Marina, V.& Morales Ortega, Ausencio& Santander González, Abimelec& Peralta-Zaragoza, Oscar& Burguete-García, A. I.…[et al.]. 2014. The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043625
Modern Language Association (MLA)
García Paz, Flor…[et al.]. The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043625
American Medical Association (AMA)
García Paz, Flor& Madrid-Marina, V.& Morales Ortega, Ausencio& Santander González, Abimelec& Peralta-Zaragoza, Oscar& Burguete-García, A. I.…[et al.]. The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043625