Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi
Joint Authors
Oliveira, Gabriel M.
Dominguez, Mariana Ribeiro
Ersching, Jonatan
Vasconcelos, José Ronnie
Araújo, Adriano Fernando
Vasconcelos, Juliana Fraga
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Bruna-Romero, Oscar
Soares, Milena Botelho
Rodrigues, Mauricio M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite.
Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian).
Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites.
The prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain.
Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block.
Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T.
cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.
American Psychological Association (APA)
Araújo, Adriano Fernando& Oliveira, Gabriel M.& Vasconcelos, Juliana Fraga& Ersching, Jonatan& Dominguez, Mariana Ribeiro& Vasconcelos, José Ronnie…[et al.]. 2014. Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043669
Modern Language Association (MLA)
Araújo, Adriano Fernando…[et al.]. Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi. Mediators of Inflammation No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043669
American Medical Association (AMA)
Araújo, Adriano Fernando& Oliveira, Gabriel M.& Vasconcelos, Juliana Fraga& Ersching, Jonatan& Dominguez, Mariana Ribeiro& Vasconcelos, José Ronnie…[et al.]. Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043669