Bisphenol A Alters β -hCG and MIF Release by Human Placenta: An In Vitro Study to Understand the Role of Endometrial Cells
Joint Authors
Wasniewski, Tomasz
Szóstek, Anna Z.
Carotenuto, C.
Romagnoli, R.
Paulesu, Luana
Skarzynski, Dariusz J.
Ietta, Francesca
Mannelli, Chiara
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success.
This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA).
As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the role of the endometrium in BPA effects on the placenta.
To this end, in vitro decidualized stromal cells were exposed to BPA 1 nM, and their conditioned medium (diluted 1 : 2) was used on chorionic villous explants from human placenta.
Parallel cultures of placental explants were directly exposed to 0.5 nM BPA while, control cultures were exposed to the vehicle (EtOH 0.1%).
After 24–48 h, culture medium from BPA-treated and control cultures was assayed for concentration of hormone human Chorionic Gonadotropin (β-hCG) and cytokine Macrophage Migration Inhibitory Factor (MIF).
The results showed that direct exposure to BPA stimulated the release of both MIF and β-hCG.
These effects were abolished/diminished in placental cultures exposed to endometrial cell-conditioned medium.
GM-MS analysis revealed that endometrial cells retain BPA, thus reducing the availability of this chemical for the placenta.
The data obtained highlight the importance of in vitro models including the maternal component in reproducing the effects of environmental chemicals on human fetus/placenta.
American Psychological Association (APA)
Mannelli, Chiara& Ietta, Francesca& Carotenuto, C.& Romagnoli, R.& Szóstek, Anna Z.& Wasniewski, Tomasz…[et al.]. 2014. Bisphenol A Alters β -hCG and MIF Release by Human Placenta: An In Vitro Study to Understand the Role of Endometrial Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043694
Modern Language Association (MLA)
Mannelli, Chiara…[et al.]. Bisphenol A Alters β -hCG and MIF Release by Human Placenta: An In Vitro Study to Understand the Role of Endometrial Cells. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043694
American Medical Association (AMA)
Mannelli, Chiara& Ietta, Francesca& Carotenuto, C.& Romagnoli, R.& Szóstek, Anna Z.& Wasniewski, Tomasz…[et al.]. Bisphenol A Alters β -hCG and MIF Release by Human Placenta: An In Vitro Study to Understand the Role of Endometrial Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043694