The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence
Joint Authors
Torres, Ana Catarina
Diniz, Patricia
Batista, Mariana
Lopes-da-Costa, Luis
Kowalczyk-Zieba, Ilona
Wocławek-Potocka, Izabela
Boruszewska, Dorota
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In the present study we examined whether LPA can be synthesized and act during in vitro maturation of bovine cumulus oocyte complexes (COCs).
We found transcription of genes coding for enzymes of LPA synthesis pathway (ATX and PLA2) and of LPA receptors (LPAR 1–4) in bovine oocytes and cumulus cells, following in vitro maturation.
COCs were matured in vitro in presence or absence of LPA ( 10 − 5 M) for 24 h.
Supplementation of maturation medium with LPA increased mRNA abundance of FST and GDF9 in oocytes and decreased mRNA abundance of CTSs in cumulus cells.
Additionally, oocytes stimulated with LPA had higher transcription levels of BCL2 and lower transcription levels of BAX resulting in the significantly lower BAX/BCL2 ratio.
Blastocyst rates on day 7 were similar in the control and the LPA-stimulated COCs.
Our study demonstrates for the first time that bovine COCs are a potential source and target of LPA action.
We postulate that LPA exerts an autocrine and/or paracrine signaling, through several LPARs, between the oocyte and cumulus cells.
LPA supplementation of maturation medium improves COC quality, and although this was not translated into an enhanced in vitro development until the blastocyst stage, improved oocyte competence may be relevant for subsequent in vivo survival.
American Psychological Association (APA)
Boruszewska, Dorota& Torres, Ana Catarina& Kowalczyk-Zieba, Ilona& Diniz, Patricia& Batista, Mariana& Lopes-da-Costa, Luis…[et al.]. 2014. The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043707
Modern Language Association (MLA)
Boruszewska, Dorota…[et al.]. The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043707
American Medical Association (AMA)
Boruszewska, Dorota& Torres, Ana Catarina& Kowalczyk-Zieba, Ilona& Diniz, Patricia& Batista, Mariana& Lopes-da-Costa, Luis…[et al.]. The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043707
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043707