Lysophosphatidic Acid Signaling in Late Cleavage and Blastocyst Stage Bovine Embryos
Joint Authors
Sinderewicz, Emilia
Torres, Ana Catarina
Diniz, Patricia
Batista, Mariana
Lopes-da-Costa, Luis
Saulnier-Blache, Jean Sebastian
Kowalczyk-Zieba, Ilona
Wocławek-Potocka, Izabela
Boruszewska, Dorota
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Lysophosphatidic acid (LPA) is a known cell signaling lipid mediator in reproductive tissues.
In the cow, LPA is involved in luteal and early pregnancy maintenance.
Here, we evaluated the presence and role of LPA in bovine early embryonic development.
In relevant aspects, bovine embryos reflect more closely the scenario occurring in human embryos than the mouse model.
Transcription of mRNA and protein expression of enzymes involved in LPA synthesis (ATX and cPLA2) and of LPA receptors (LPAR1–4) were detected in Days 5 and 8 in vitro produced embryos.
Embryonic LPA production into culture medium was also detected at both stages of development.
Supplementation of culture medium with LPA (10−5 M) between Days 2 and 8 had no effect on embryo yield and quality and on blastocyst relative mRNA abundance of genes involved in prostaglandin synthesis (PTGS2, PGES, and PGFS) and steroidogenesis (3βHSD).
However, LPA treatment affected transcription levels of embryo quality markers, decreasing BAX (apoptotic) and increasing BCL2 (antiapoptotic) and IGF2R (growth marker) gene transcription levels.
Blastocyst transcription of OCT4 (pluripotency marker) was not affected by LPA stimulation.
In conclusion, LPA is an early bovine embryonic autocrine/paracrine signaling mediator, and LPA action may be relevant in early embryo-maternal interactions leading to embryonic survival.
American Psychological Association (APA)
Torres, Ana Catarina& Boruszewska, Dorota& Batista, Mariana& Kowalczyk-Zieba, Ilona& Diniz, Patricia& Sinderewicz, Emilia…[et al.]. 2014. Lysophosphatidic Acid Signaling in Late Cleavage and Blastocyst Stage Bovine Embryos. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043709
Modern Language Association (MLA)
Torres, Ana Catarina…[et al.]. Lysophosphatidic Acid Signaling in Late Cleavage and Blastocyst Stage Bovine Embryos. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043709
American Medical Association (AMA)
Torres, Ana Catarina& Boruszewska, Dorota& Batista, Mariana& Kowalczyk-Zieba, Ilona& Diniz, Patricia& Sinderewicz, Emilia…[et al.]. Lysophosphatidic Acid Signaling in Late Cleavage and Blastocyst Stage Bovine Embryos. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043709
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043709