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Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study
Joint Authors
Ferreira-Dias, Graça
Galvão, António M.
Szóstek, Anna Z.
Tramontano, Angela
Rebordão, Maria Rosa
Amaral, Ana
Bravo, Pedro Pinto
Mollo, Antonio
Skarzynski, Dariusz J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-13
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Metabolic hormones have been associated with reproductive function modulation.
Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate the role of LEP and GHR on progesterone (P4), prostaglandin (PG) E2 and PGF2α, nitric oxide (nitrite), tumor necrosis factor-α (TNF); macrophage migration inhibitory factor (MIF) secretion, and on angiogenic activity (BAEC proliferation), in equine corpus luteum (CL) from early and mid-luteal stages.
LEPR expression was decreased in late CL, while GHR/Ghr-R1A system was increased in the same stage.
Regarding secretory activity, GHR decreased P4 in early CL, but increased PGF2α, nitrite and TNF in mid CL.
Conversely, LEP increased P4, PGE2, angiogenic activity, MIF, TNF and nitrite during early CL, in a dose-dependent manner.
The in vitro effect of LEP on secretory activity was reverted by GHR, when both factors acted together.
The present results evidence the presence of LEP and GHR systems in the equine CL.
Moreover, we suggest that LEP and GHR play opposing roles in equine CL regulation, with LEP supporting luteal establishment and GHR promoting luteal regression.
Finally, a dose-dependent luteotrophic effect of LEP was demonstrated.
American Psychological Association (APA)
Galvão, António M.& Tramontano, Angela& Rebordão, Maria Rosa& Amaral, Ana& Bravo, Pedro Pinto& Szóstek, Anna Z.…[et al.]. 2014. Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043711
Modern Language Association (MLA)
Galvão, António M.…[et al.]. Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study. Mediators of Inflammation No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043711
American Medical Association (AMA)
Galvão, António M.& Tramontano, Angela& Rebordão, Maria Rosa& Amaral, Ana& Bravo, Pedro Pinto& Szóstek, Anna Z.…[et al.]. Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043711
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043711