![](/images/graphics-bg.png)
Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via CalciumCalmodulin-Dependent Protein Kinase 4
Joint Authors
Kim, Ji-Hee
Park, Ga-Young
Bang, Soo Young
Park, Sun Young
Bae, Soo-Kyung
Kim, YoungHee
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Crocin is a water-soluble carotenoid pigment that is primarily used in various cuisines as a seasoning and coloring agent, as well as in traditional medicines for the treatment of edema, fever, and hepatic disorder.
In this study, we demonstrated that crocin markedly induces the expression of heme oxygenase-1 (HO-1) which leads to an anti-inflammatory response.
Crocin inhibited inducible nitric oxide synthase (iNOS) expression and nitric oxide production via downregulation of nuclear factor kappa B activity in lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophages.
These effects were abrogated by blocking of HO-1 expression or activity.
Crocin also induced Ca2+ mobilization from intracellular pools and phosphorylation of Ca2+/calmodulin-dependent protein kinase 4 (CAMK4).
CAMK4 knockdown and kinase-dead mutant inhibited crocin-mediated HO-1 expression, Nrf2 activation, and phosphorylation of Akt, indicating that HO-1 expression is mediated by CAMK4 and that Akt is a downstream mediator of CAMK4 in crocin signaling.
Moreover, crocin-mediated suppression of iNOS expression was blocked by CAMK4 inhibition.
Overall, these results suggest that crocin suppresses LPS-stimulated expression of iNOS by inducing HO-1 expression via Ca2+/calmodulin-CAMK4-PI3K/Akt-Nrf2 signaling cascades.
Our findings provide a novel molecular mechanism for the inhibitory effects of crocin against endotoxin-mediated inflammation.
American Psychological Association (APA)
Kim, Ji-Hee& Park, Ga-Young& Bang, Soo Young& Park, Sun Young& Bae, Soo-Kyung& Kim, YoungHee. 2014. Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via CalciumCalmodulin-Dependent Protein Kinase 4. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043766
Modern Language Association (MLA)
Kim, Ji-Hee…[et al.]. Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via CalciumCalmodulin-Dependent Protein Kinase 4. Mediators of Inflammation No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1043766
American Medical Association (AMA)
Kim, Ji-Hee& Park, Ga-Young& Bang, Soo Young& Park, Sun Young& Bae, Soo-Kyung& Kim, YoungHee. Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via CalciumCalmodulin-Dependent Protein Kinase 4. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043766
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043766