Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells
Joint Authors
Zhou, Yan
Chen, Song
Ding, Ran
Gao, Xiangdong
Zhang, Xian
Zhu, Rui
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
YCP, as a kind of natural polysaccharides from the mycelium of marine filamentous fungus Phoma herbarum YS4108, has great antitumor potential via enhancement of host immune response, but little is known about the molecular mechanisms.
In the present study, we mainly focused on the effects and mechanisms of YCP on the specific immunity mediated by dendritic cells (DCs) and T cells.
T cell /DC activation-related factors including interferon- (IFN-) γ, interleukin-12 (IL-12), and IL-4 were examined with ELISA.
Receptor knock-out mice and fluorescence-activated cell sorting are used to analyze the YCP-binding receptor of T cells and DCs.
RT-PCR is utilized to measure MAGE-A3 for analyzing the tumor-specific killing effect.
In our study, we demonstrated YCP can provide the second signal for T cell activation, proliferation, and IFN-γ production through binding to toll-like receptor- (TLR-) 2 and TLR-4.
YCP could effectively promote IL-12 secretion and expression of markers (CD80, CD86, and MHC II) via TLR-4 on DCs.
Antigen-specific immunity against mouse melanoma cells was strengthened through the activation of T cells and the enhancement of capacity of DCs by YCP.
The data supported that YCP can exhibit specific immunomodulatory capacity mediated by T cells and DCs.
American Psychological Association (APA)
Chen, Song& Ding, Ran& Zhou, Yan& Zhang, Xian& Zhu, Rui& Gao, Xiangdong. 2014. Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043770
Modern Language Association (MLA)
Chen, Song…[et al.]. Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells. Mediators of Inflammation No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043770
American Medical Association (AMA)
Chen, Song& Ding, Ran& Zhou, Yan& Zhang, Xian& Zhu, Rui& Gao, Xiangdong. Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043770