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Hyperthermia Differently Affects Connexin43 Expression and Gap Junction Permeability in Skeletal Myoblasts and HeLa Cells
Joint Authors
Antanavičiūtė, Ieva
Mildažienė, Vida
Stankevičius, Edgaras
Herdegen, Thomas
Skeberdis, Vytenis Arvydas
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Stress kinases can be activated by hyperthermia and modify the expression level and properties of membranous and intercellular channels.
We examined the role of c-Jun NH2-terminal kinase (JNK) in hyperthermia-induced changes of connexin43 (Cx43) expression and permeability of Cx43 gap junctions (GJs) in the rabbit skeletal myoblasts (SkMs) and Cx43-EGFP transfected HeLa cells.
Hyperthermia (42°C for 6 h) enhanced the activity of JNK and its target, the transcription factor c-Jun, in both SkMs and HeLa cells.
In SkMs, hyperthermia caused a 3.2-fold increase in the total Cx43 protein level and enhanced the efficacy of GJ intercellular communication (GJIC).
In striking contrast, hyperthermia reduced the total amount of Cx43 protein, the number of Cx43 channels in GJ plaques, the density of hemichannels in the cell membranes, and the efficiency of GJIC in HeLa cells.
Both in SkMs and HeLa cells, these changes could be prevented by XG-102, a JNK inhibitor.
In HeLa cells, the changes in Cx43 expression and GJIC under hyperthermic conditions were accompanied by JNK-dependent disorganization of actin cytoskeleton stress fibers while in SkMs, the actin cytoskeleton remained intact.
These findings provide an attractive model to identify the regulatory players within signalosomes, which determine the cell-dependent outcomes of hyperthermia.
American Psychological Association (APA)
Antanavičiūtė, Ieva& Mildažienė, Vida& Stankevičius, Edgaras& Herdegen, Thomas& Skeberdis, Vytenis Arvydas. 2014. Hyperthermia Differently Affects Connexin43 Expression and Gap Junction Permeability in Skeletal Myoblasts and HeLa Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1043781
Modern Language Association (MLA)
Antanavičiūtė, Ieva…[et al.]. Hyperthermia Differently Affects Connexin43 Expression and Gap Junction Permeability in Skeletal Myoblasts and HeLa Cells. Mediators of Inflammation No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1043781
American Medical Association (AMA)
Antanavičiūtė, Ieva& Mildažienė, Vida& Stankevičius, Edgaras& Herdegen, Thomas& Skeberdis, Vytenis Arvydas. Hyperthermia Differently Affects Connexin43 Expression and Gap Junction Permeability in Skeletal Myoblasts and HeLa Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1043781
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043781