CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice
Joint Authors
Chelmonska-Soyta, Anna
Maj, Tomasz
Slawek, Anna
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Immune phenomena during the preimplantation period of pregnancy are poorly understood.
The aim of our study was to assess the capacity for antigen presentation of splenic antigen-presenting cells (APCs) derived from pregnant and pseudopregnant mice in in vitro conditions.
Therefore, sorted CD11c+ dendritic cells and macrophages F4/80+ and CD11b+ presenting ovalbumin (OVA) were cocultured with CD4+ T cells derived from OT-II mice’s (C57BL6/J-Tg(TcraTcrb)1100Mjb/J) spleen.
After 132 hours of cell culture, proliferation of lymphocytes (ELISA-BrdU), activation of these cells (flow cytometry), cytokine profile (ELISA), and influence of costimulatory molecules blocking on these parameters were measured.
We did not detect any differences in regulation of Th1/Th2 cytokine balance.
CD86 seems to be the main costimulatory molecule involved in the proliferation response but CD80 is the main costimulatory molecule influencing cytokine secretion in pregnant mice.
In conclusion, this study showed that CD80 and CD86 costimulatory molecules regulate OT-II CD4+ T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice.
The implications of these changes still remain unclear.
American Psychological Association (APA)
Maj, Tomasz& Slawek, Anna& Chelmonska-Soyta, Anna. 2014. CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043801
Modern Language Association (MLA)
Maj, Tomasz…[et al.]. CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice. Mediators of Inflammation No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043801
American Medical Association (AMA)
Maj, Tomasz& Slawek, Anna& Chelmonska-Soyta, Anna. CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043801