The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat
Joint Authors
Zong, Shaohui
Zeng, Gaofeng
Fang, Ye
Peng, Jinzhen
Tao, Yong
Li, Keke
Zhao, Jingmin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Study Design.
In this study, we investigated the role of IL-17 via activation of STAT3 in the pathophysiology of SCI.
Objective.
The purpose of the experiments is to study the expression of IL-17 and related cytokines via STAT3 signaling pathways, which is caused by the acute inflammatory response following SCI in different periods via establishing an acute SCI model in rat.
Methods.
Basso, Beattie, and Bresnahan hind limb locomotor rating scale was used to assess the rat hind limb motor function.
Immunohistochemistry was used to determine the expression levels of IL-17 and p-STAT3 in spinal cord tissues.
Western blotting analysis was used to determine the protein expression of p-STAT3 in spinal cord tissue.
RT-PCR was used to analyze the mRNA expression of IL-17 and IL-23p19 in the spleen tissue.
ELISA was used to determine the peripheral blood serum levels of IL-6, IL-21, and IL-23.
Results.
Compared to the sham-operated group, the expression levels of IL-17, p-STAT3, IL-6, IL-21, and IL-23 were significantly increased and peaked at 24 h after SCI.
The increased levels of cytokines were correlated with the SCI disease stages.
Conclusion.
IL-17 may play an important role in promoting spinal cord neuroinflammation after SCI via activation of STAT3.
American Psychological Association (APA)
Zong, Shaohui& Zeng, Gaofeng& Fang, Ye& Peng, Jinzhen& Tao, Yong& Li, Keke…[et al.]. 2014. The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043806
Modern Language Association (MLA)
Zong, Shaohui…[et al.]. The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043806
American Medical Association (AMA)
Zong, Shaohui& Zeng, Gaofeng& Fang, Ye& Peng, Jinzhen& Tao, Yong& Li, Keke…[et al.]. The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043806