HMGB1 Localization during Experimental Periodontitis
Joint Authors
de Molon, Rafael Scaf
Cirelli, Joni Augusto
da Silva Mariano Pereira, Elyne
de Aquino, Sabrina Garcia
Nogueira, Andressa Vilas Boas
Chaves de Souza, João Antônio
Giannobile, William V.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1β and in vivo during ligature- or LPS-induced periodontitis in rats.
Material and Methods.
For the in vivo study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7–30 d following disease induction.
Bone loss and inflammation were evaluated.
HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot.
Results.
Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12 h with protein expression elevated by 24 h.
HMGB1 mRNA expression in gingival tissues was significantly increased at 15 d in the LPS-PD model and at 7 and 15 d in the ligature model.
Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods.
Conclusion.
The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli.
In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction.
American Psychological Association (APA)
Nogueira, Andressa Vilas Boas& Chaves de Souza, João Antônio& de Molon, Rafael Scaf& da Silva Mariano Pereira, Elyne& de Aquino, Sabrina Garcia& Giannobile, William V.…[et al.]. 2014. HMGB1 Localization during Experimental Periodontitis. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043826
Modern Language Association (MLA)
Nogueira, Andressa Vilas Boas…[et al.]. HMGB1 Localization during Experimental Periodontitis. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043826
American Medical Association (AMA)
Nogueira, Andressa Vilas Boas& Chaves de Souza, João Antônio& de Molon, Rafael Scaf& da Silva Mariano Pereira, Elyne& de Aquino, Sabrina Garcia& Giannobile, William V.…[et al.]. HMGB1 Localization during Experimental Periodontitis. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043826