Sustained Release of Prostaglandin E 2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca 2+-Mobilization
Joint Authors
Pimentel-Santillana, María
Través, Paqui G.
Pérez-Sen, Raquel
Delicado, Esmerilda G.
Martín-Sanz, Paloma
Miras-Portugal, María Teresa
Boscá, Lisardo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nucleotide uridine trisphosphate (UTP) released to the extracellular milieu acts as a signaling molecule via activation of specific pyrimidine receptors (P2Y).
P2Y receptors are G protein-coupled receptors expressed in many cell types.
These receptors mediate several cell responses and they are involved in intracellular calcium mobilization.
We investigated the role of the prostanoid PGE2 in P2Y signaling in mouse embryonic fibroblasts (MEFs), since these cells are involved in different ontogenic and physiopathological processes, among them is tissue repair following proinflammatory activation.
Interestingly, Ca2+-mobilization induced by UTP-dependent P2Y activation was reduced by PGE2 when this prostanoid was produced by MEFs transfected with COX-2 or when PGE2 was added exogenously to the culture medium.
This Ca2+-mobilization was important for the activation of different metabolic pathways in fibroblasts.
Moreover, inhibition of COX-2 with selective coxibs prevented UTP-dependent P2Y activation in these cells.
The inhibition of P2Y responses by PGE2 involves the activation of PKCs and PKD, a response that can be suppressed after pharmacological inhibition of these protein kinases.
In addition to this, PGE2 reduces the fibroblast migration induced by P2Y-agonists such as UTP.
Taken together, these data demonstrate that PGE2 is involved in the regulation of P2Y signaling in these cells.
American Psychological Association (APA)
Pimentel-Santillana, María& Través, Paqui G.& Pérez-Sen, Raquel& Delicado, Esmerilda G.& Martín-Sanz, Paloma& Miras-Portugal, María Teresa…[et al.]. 2014. Sustained Release of Prostaglandin E 2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca 2+-Mobilization. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043837
Modern Language Association (MLA)
Pimentel-Santillana, María…[et al.]. Sustained Release of Prostaglandin E 2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca 2+-Mobilization. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043837
American Medical Association (AMA)
Pimentel-Santillana, María& Través, Paqui G.& Pérez-Sen, Raquel& Delicado, Esmerilda G.& Martín-Sanz, Paloma& Miras-Portugal, María Teresa…[et al.]. Sustained Release of Prostaglandin E 2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca 2+-Mobilization. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043837
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043837