Inhibition of Ocular Aldose Reductase by a New Benzofuroxane Derivative Ameliorates Rat Endotoxic Uveitis
Joint Authors
Maione, Sabatino
Cosconati, Sandro
Ferraraccio, Franca
Di Filippo, Clara
Zippo, M. V.
Maisto, R.
Trotta, M. C.
Ferraro, B.
Sartini, S.
Gesualdo, C.
Rossi, S.
La Motta, Concettina
Novellino, Ettore
D'Amico, Michele
Simonelli, Francesca
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The study investigated the effects of the aldose reductase (AR) inhibitor benzofuroxane derivative 5(6)-(benzo[d]thiazol-2-ylmethoxy) benzofuroxane (herein referred to as BF-5m) on the biochemical and tissue alterations induced by endotoxic uveitis in rats.
BF-5m has been administered directly into the vitreous, in order to assess the expression and levels of (i) inflammatory markers such as the ocular ubiquitin-proteasome system, NF-κB, TNF-α, and MCP-1; (ii) prooxidant and antioxidant markers such as nitrotyrosine, manganese superoxide dismutase (MnSOD), and glutathione peroxidase (GPX); (iii) apoptotic/antiapoptotic factors caspases and Bcl-xl; (iv) markers of endothelial progenitor cells (EPCs) recruitment such as CD34 and CD117.
5 μL of BF-5m (0.01; 0.05; and 0.1 μM) into the right eye decreased in a dose-dependent manner the LPS-induced inflammation of the eye, reporting a clinical score 1.
It reduced the ocular levels of ubiquitin, 20S and 26S proteasome subunits, NF-κB subunits, TNF-α, MCP-1, and nitrotyrosine.
BF-5m ameliorated LPS-induced decrease in levels of MnSOD and GPX.
Antiapoptotic effects were seen from BF-5m by monitoring the expression of Bcl-xl, an antiapoptotic protein.
Similarly, BF-5m increased recruitment of the EPCs within the eye, as evidenced by CD34 and CD117 antibodies.
American Psychological Association (APA)
Di Filippo, Clara& Zippo, M. V.& Maisto, R.& Trotta, M. C.& Maione, Sabatino& Ferraro, B.…[et al.]. 2014. Inhibition of Ocular Aldose Reductase by a New Benzofuroxane Derivative Ameliorates Rat Endotoxic Uveitis. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043850
Modern Language Association (MLA)
Di Filippo, Clara…[et al.]. Inhibition of Ocular Aldose Reductase by a New Benzofuroxane Derivative Ameliorates Rat Endotoxic Uveitis. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043850
American Medical Association (AMA)
Di Filippo, Clara& Zippo, M. V.& Maisto, R.& Trotta, M. C.& Maione, Sabatino& Ferraro, B.…[et al.]. Inhibition of Ocular Aldose Reductase by a New Benzofuroxane Derivative Ameliorates Rat Endotoxic Uveitis. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043850
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043850