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Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
Joint Authors
Zhang, Wen-cai
Wang, Yan-ge
Zhu, Zheng-feng
Wu, Fang-qin
Peng, Yu-dong
Chen, Zhu-yue
Yang, Jin-hua
Wu, Jing-jing
Lian, Yi-tian
He, Mei-an
Wu, Tang-chun
Cheng, Long-xian
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Objective.
To investigate the role of CD4+CD25+ T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms.
Methods.
Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm2) of suspension of fine particles for 24h.
For coculture experiment, HUVECs were incubated alone, with CD4+CD25− T cells (Teff), or with Tregs in the presence of anti-CD3 monoclonal antibodies for 48 hours, and then were stimulated with or without suspension of fine particles for 24 hours.
The expression of adhesion molecules and inflammatory cytokines was examined.
Results.
Adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and inflammatory cytokines, such as interleukin (IL-) 6 and IL-8, were increased in a concentration-dependent manner.
Moreover, the adhesion of human acute monocytic leukemia cells (THP-1) to endothelial cells was increased and NF-κB activity was upregulated in HUVECs after treatment with fine particles.
However, after Tregs treatment, fine particles-induced inflammatory responses and NF-κB activation were significantly alleviated.
Transwell experiments showed that Treg-mediated suppression of HUVECs inflammatory responses impaired by fine particles required cell contact and soluble factors.
Conclusions.
Tregs could attenuate fine particles-induced inflammatory responses and NF-κB activation in HUVECs.
American Psychological Association (APA)
Zhang, Wen-cai& Wang, Yan-ge& Zhu, Zheng-feng& Wu, Fang-qin& Peng, Yu-dong& Chen, Zhu-yue…[et al.]. 2014. Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043858
Modern Language Association (MLA)
Zhang, Wen-cai…[et al.]. Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1043858
American Medical Association (AMA)
Zhang, Wen-cai& Wang, Yan-ge& Zhu, Zheng-feng& Wu, Fang-qin& Peng, Yu-dong& Chen, Zhu-yue…[et al.]. Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043858