Upregulating Nonneuronal Cholinergic Activity Decreases TNF Release from Lipopolysaccharide-Stimulated RAW264.7 Cells
Joint Authors
Du, Ming-Hua
Lv, Yi
Lu, Jiangyang
Dong, Ning
Liu, Qian
Zhang, Huiping
Hu, Sen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Nonneuronal cholinergic system plays a primary role in maintaining homeostasis.
It has been proved that endogenous neuronal acetylcholine (ACh) could play an anti-inflammatory role, and exogenous cholinergic agonists could weaken macrophages inflammatory response to lipopolysaccharide (LPS) stimulation through activation of α 7 subunit-containing nicotinic acetylcholine receptor ( α 7nAChR).
We assumed that nonneuronal cholinergic system existing in macrophages could modulate inflammation through autocrine ACh and expressed α 7nAChR on the cells.
Therefore, we explored whether LPS continuous stimulation could upregulate the nonneuronal cholinergic activity in macrophages and whether increasing autocrine ACh could decrease TNF release from the macrophages.
The results showed that, in RAW264.7 cells incubated with LPS for 20 hours, the secretion of ACh was significantly decreased at 4 h and then gradually increased, accompanied with the enhancement of α 7nAChR expression level.
The release of TNF was greatly increased from RAW264.7 cells at 4 h and 8 h exposure to LPS; however, it was suppressed at 20 h.
Upregulating choline acetyltransferase (ChAT) expression through ChAT gene transfection could enhance ACh secretion and reduce TNF release from the infected RAW264.
7cells.
The results indicated that LPS stimulation could modulate the activity of nonneuronal cholinergic system of RAW264.7 cells.
Enhancing autocrine ACh production could attenuate TNF release from RAW264.7 cells.
American Psychological Association (APA)
Lv, Yi& Hu, Sen& Lu, Jiangyang& Dong, Ning& Liu, Qian& Du, Ming-Hua…[et al.]. 2014. Upregulating Nonneuronal Cholinergic Activity Decreases TNF Release from Lipopolysaccharide-Stimulated RAW264.7 Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043861
Modern Language Association (MLA)
Lv, Yi…[et al.]. Upregulating Nonneuronal Cholinergic Activity Decreases TNF Release from Lipopolysaccharide-Stimulated RAW264.7 Cells. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043861
American Medical Association (AMA)
Lv, Yi& Hu, Sen& Lu, Jiangyang& Dong, Ning& Liu, Qian& Du, Ming-Hua…[et al.]. Upregulating Nonneuronal Cholinergic Activity Decreases TNF Release from Lipopolysaccharide-Stimulated RAW264.7 Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043861