An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells
Joint Authors
Kumawat, Ashok Kumar
Tysk, Curt
Hultgren Hörnquist, Elisabeth
Nyhlin, Nils
Wickbom, Anna
Bohr, Johan
Hultgren, Olof
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Soluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut.
We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation of T cells.
Peripheral blood CD4+ T cells from healthy donors were polyclonally activated in the presence of conditioned medium (CM) generated from denuded biopsies (DNB) or isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients compared to noninflamed controls, to determine proliferation and secretion of cytokines involved in T-cell differentiation.
Compared to controls, we observed significantly increased production of the proinflammatory cytokines IFN-γ, IL-17A, IL-6, and IL-1β and the anti-inflammatory cytokines IL-4 and IL-10 in the presence of CC-DNB-CM.
The most pronounced effect of CC-LPMC-CM on peripheral CD4+ T cells was a trend towards increased production of IL-17A and IL-10.
A trend towards reduced inhibition of T-cell proliferation was noted in the presence of CC-DNB-CM.
In conclusion, our in vitro model reveals implications of soluble factors from CC colonic mucosa on peripheral T cells, enhancing their production of both pro- and anti-inflammatory cytokines.
American Psychological Association (APA)
Kumawat, Ashok Kumar& Nyhlin, Nils& Wickbom, Anna& Tysk, Curt& Bohr, Johan& Hultgren, Olof…[et al.]. 2014. An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043864
Modern Language Association (MLA)
Kumawat, Ashok Kumar…[et al.]. An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells. Mediators of Inflammation No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043864
American Medical Association (AMA)
Kumawat, Ashok Kumar& Nyhlin, Nils& Wickbom, Anna& Tysk, Curt& Bohr, Johan& Hultgren, Olof…[et al.]. An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043864