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Increased Expression of Chitinase 3-Like 1 in Aorta of Patients with Atherosclerosis and Suppression of Atherosclerosis in Apolipoprotein E-Knockout Mice by Chitinase 3-Like 1 Gene Silencing
Joint Authors
Gong, Zushun
Xing, Shanshan
Zheng, Fei
Xing, Qichong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Introduction.
The purpose of this study was to investigate the changes of chitinase 3-like 1 (CHI3L1) in the aorta of patients with coronary atherosclerosis and to determine whether inhibition of CHI3L1 by lentivirus-mediated RNA interference could stabilize atherosclerotic plaques in apolipoprotein E-knockout (ApoE−/−) mice.
Methods.
We collected discarded aortic specimens from patients undergoing coronary artery bypass graft surgery and renal arterial tissues from kidney donors.
A lentivirus carrying small interfering RNA targeting the expression of CHI3L1 was constructed.
Fifty ApoE−/− mice were divided into control group and CHI3L1 gene silenced group.
A constrictive collar was placed around carotid artery to induce plaques formation.
Then lentivirus was transfected into carotid plaques.
Results.
We found that CHI3L1 was overexpressed in aorta of patients with atherosclerosis and its expression was correlated with the atherosclerotic risk factors.
After lentivirus transduction, mRNA and protein expression of CHI3L1 were attenuated in carotid plaques, leading to reduced plaque content of lipids and macrophages, and increased plaque content of collagen and smooth muscle cells.
Moreover, CHI3L1 gene silencing downregulated the expression of local proinflammatory mediators.
Conclusions.
CHI3L1 is overexpressed in aorta from patients with atherosclerosis and the lentivirus-mediated CHI3L1 gene silencing could represent a new strategy to inhibit plaques progression.
American Psychological Association (APA)
Gong, Zushun& Xing, Shanshan& Zheng, Fei& Xing, Qichong. 2014. Increased Expression of Chitinase 3-Like 1 in Aorta of Patients with Atherosclerosis and Suppression of Atherosclerosis in Apolipoprotein E-Knockout Mice by Chitinase 3-Like 1 Gene Silencing. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043875
Modern Language Association (MLA)
Gong, Zushun…[et al.]. Increased Expression of Chitinase 3-Like 1 in Aorta of Patients with Atherosclerosis and Suppression of Atherosclerosis in Apolipoprotein E-Knockout Mice by Chitinase 3-Like 1 Gene Silencing. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043875
American Medical Association (AMA)
Gong, Zushun& Xing, Shanshan& Zheng, Fei& Xing, Qichong. Increased Expression of Chitinase 3-Like 1 in Aorta of Patients with Atherosclerosis and Suppression of Atherosclerosis in Apolipoprotein E-Knockout Mice by Chitinase 3-Like 1 Gene Silencing. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043875
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043875