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Myocardial Gene Expression of T-bet, GATA-3, Ror- γ t, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response
Joint Authors
Cunha-Neto, Edecio
Bocchi, E. A.
Nogueira, Luciana Gabriel
Santos, Ronaldo Honorato Barros
Fiorelli, Alfredo Inácio
Mairena, Eliane Conti
Benvenuti, Luiz Alberto
Stolf, Noedir Antonio
Kalil, Jorge
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Chronic Chagas disease cardiomyopathy (CCC), a late consequence of Trypanosoma cruzi infection, is an inflammatory cardiomyopathy with prognosis worse than those of noninflammatory etiology (NIC).
Although the T cell-rich myocarditis is known to play a pathogenetic role, the relative contribution of each of the functional T cell subsets has never been thoroughly investigated.
We therefore assessed gene expression of cytokines and transcription factors involved in differentiation and effector function of each functional T cell subset ( T H 1/ T H 2/ T H 17/Treg) in CCC, NIC, and heart donor myocardial samples.
Methods and Results.
Quantitative PCR showed markedly upregulated expression of IFN-γ and transcription factor T-bet, and minor increases of GATA-3; FoxP3 and CTLA-4; IL-17 and IL-18 in CCC as compared with NIC samples.
Conversely, cytokines expressed by T H 2 cells (IL-4, IL-5, and IL-13) or associated with Treg (TGF-β and IL-10) were not upregulated in CCC myocardium.
Expression of T H 1-related genes such as T-bet, IFN-γ , and IL-18 correlated with ventricular dilation, FoxP3, and CTLA-4.
Conclusions.
Results are consistent with a strong local T H 1-mediated response in most samples, possibly associated with pathological myocardial remodeling, and a proportionally smaller FoxP3+CTLA4+ Treg cell population, which is unable to completely curb IFN-γ production in CCC myocardium, therefore fueling inflammation.
American Psychological Association (APA)
Nogueira, Luciana Gabriel& Santos, Ronaldo Honorato Barros& Fiorelli, Alfredo Inácio& Mairena, Eliane Conti& Benvenuti, Luiz Alberto& Bocchi, E. A.…[et al.]. 2014. Myocardial Gene Expression of T-bet, GATA-3, Ror- γ t, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043891
Modern Language Association (MLA)
Nogueira, Luciana Gabriel…[et al.]. Myocardial Gene Expression of T-bet, GATA-3, Ror- γ t, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043891
American Medical Association (AMA)
Nogueira, Luciana Gabriel& Santos, Ronaldo Honorato Barros& Fiorelli, Alfredo Inácio& Mairena, Eliane Conti& Benvenuti, Luiz Alberto& Bocchi, E. A.…[et al.]. Myocardial Gene Expression of T-bet, GATA-3, Ror- γ t, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043891
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043891