Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity
Joint Authors
Monk, Jennifer M.
Turk, Harmony F.
Fan, Yang-Yi
Callaway, Evelyn
Weeks, Brad
Yang, Peiying
Chapkin, Robert S.
McMurray, David N.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
During colitis, activation of two inflammatory T cell subsets, Th17 and Th1 cells, promotes ongoing intestinal inflammatory responses.
n-6 polyunsaturated fatty acid- (PUFA-) derived eicosanoids, such as prostaglandin E2 (PGE2), promote Th17 cell-mediated inflammation, while n-3 PUFA antagonize both Th17 and Th1 cells and suppress PGE2 levels.
We utilized two genetic mouse models, which differentially antagonize PGE2 levels, to examine the effect on Th17 cells and disease outcomes in trinitrobenzene sulfonic acid- (TNBS-) induced colitis.
Fat-1 mice contain the ω 3 desaturase gene from C.
elegans and synthesize n-3 PUFA de novo, thereby reducing the biosynthesis of n-6 PUFA-derived eicosanoids.
In contrast, Fads1 Null mice contain a disrupted Δ 5 desaturase gene and produce lower levels of n-6 PUFA-derived eicosanoids.
Compared to Wt littermates, Fat-1 and Fads1 Null mice exhibited a similar colitic phenotype characterized by reduced colonic mucosal inflammatory eicosanoid levels and mRNA expression of Th17 cell markers (IL-17A, ROR γ τ , and IL-23), decreased percentages of Th17 cells and, improved colon injury scores ( P ≤ 0.05 ).
Thus, during colitis, similar outcomes were obtained in two genetically distinct models, both of which antagonize PGE2 levels via different mechanisms.
Our data highlight the critical impact of n-6 PUFA-derived eicosanoids in the promotion of Th17 cell-mediated colonic inflammation.
American Psychological Association (APA)
Monk, Jennifer M.& Turk, Harmony F.& Fan, Yang-Yi& Callaway, Evelyn& Weeks, Brad& Yang, Peiying…[et al.]. 2014. Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043894
Modern Language Association (MLA)
Monk, Jennifer M.…[et al.]. Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity. Mediators of Inflammation No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1043894
American Medical Association (AMA)
Monk, Jennifer M.& Turk, Harmony F.& Fan, Yang-Yi& Callaway, Evelyn& Weeks, Brad& Yang, Peiying…[et al.]. Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043894
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043894