LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis
Joint Authors
Jäger, Andreas
Götz, Werner
Gölz, L.
Memmert, S.
Rath-Deschner, B.
Appel, T.
Frede, S.
Baumgarten, Georg
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-13
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Oxidative stress is characterized by an accumulation of reactive oxygen species (ROS) and plays a key role in the progression of inflammatory diseases.
We hypothesize that hypoxic and inflammatory events induce oxidative stress in the periodontal ligament (PDL) by activating NOX4.
Human primary PDL fibroblasts were stimulated with lipopolysaccharide from Porphyromonas gingivalis (LPS-PG), a periodontal pathogen bacterium under normoxic and hypoxic conditions.
By quantitative PCR, immunoblot, immunostaining, and a specific ROS assay we determined the amount of NOX4, ROS, and several redox systems.
Healthy and inflamed periodontal tissues were collected to evaluate NOX4 and redox systems by immunohistochemistry.
We found significantly increased NOX4 levels after hypoxic or inflammatory stimulation in PDL cells ( P < 0.001 ) which was even more pronounced after combination of the stimuli.
This was accompanied by a significant upregulation of ROS and catalase ( P < 0.001 ).
However, prolonged incubation with both stimuli induced a reduction of catalase indicating a collapse of the protective machinery favoring ROS increase and the progression of inflammatory oral diseases.
Analysis of inflamed tissues confirmed our hypothesis.
In conclusion, we demonstrated that the interplay of NOX4 and redox systems is crucial for ROS formation which plays a pivotal role during oral diseases.
American Psychological Association (APA)
Gölz, L.& Memmert, S.& Rath-Deschner, B.& Jäger, Andreas& Appel, T.& Baumgarten, Georg…[et al.]. 2014. LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043954
Modern Language Association (MLA)
Gölz, L.…[et al.]. LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis. Mediators of Inflammation No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043954
American Medical Association (AMA)
Gölz, L.& Memmert, S.& Rath-Deschner, B.& Jäger, Andreas& Appel, T.& Baumgarten, Georg…[et al.]. LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043954