Treatment with Anti-EGF Ab Ameliorates Experimental Autoimmune Encephalomyelitis via Induction of Neurogenesis and Oligodendrogenesis
Joint Authors
Mausner-Fainberg, Karin
Karni, Arnon
Levy-Amir, Yifat
Source
Multiple Sclerosis International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
The neural stem cells (NSCs) migrate to the damaged sites in multiple sclerosis (MS) and in experimental autoimmune encephalomyelitis (EAE).
However, the differentiation into neurons or oligodendrocytes is blocked.
Epidermal growth factor (EGF) stimulates NSC proliferation and mobilization to demyelinated lesions but also induces astrogenesis and glial scar.
Objective.
To examine the clinical and histopathological effects of EGF neutralization on EAE.
Methods.
EAE-induced SJL mice were intravenously treated with either anti-EGF neutralizing antibody (Ab) or isotype control or PBS.
On day 9 after immunization, 3 mice of each group were daily treated for 9 days with BrdU and then sacrificed for immunohistochemical analysis.
Results.
Treatment with anti-EGF Ab significantly ameliorated EAE symptoms during the second relapse.
Anti-EGF Ab induced a shift from BrdU+GFAP+ NSCs to BrdU+DCX+ neuroblasts in the subventricular zone (SVZ), increased BrdU+NeuN+ neurons in the granular cell layer of the dentate gyrus, and increased BrdU+O4+ oligodendrocytes in the SVZ.
There was no change in the inflammatory infiltrates in response to anti-EGF Ab.
Conclusions.
Therapy with anti-EGF Ab ameliorates EAE via induction of neurogenesis and oligodendrogenesis.
No immunosuppressive effect was found.
Further investigation is needed to support these notions of beneficial effect of anti-EGF Ab in MS.
American Psychological Association (APA)
Levy-Amir, Yifat& Mausner-Fainberg, Karin& Karni, Arnon. 2014. Treatment with Anti-EGF Ab Ameliorates Experimental Autoimmune Encephalomyelitis via Induction of Neurogenesis and Oligodendrogenesis. Multiple Sclerosis International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1046621
Modern Language Association (MLA)
Levy-Amir, Yifat…[et al.]. Treatment with Anti-EGF Ab Ameliorates Experimental Autoimmune Encephalomyelitis via Induction of Neurogenesis and Oligodendrogenesis. Multiple Sclerosis International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1046621
American Medical Association (AMA)
Levy-Amir, Yifat& Mausner-Fainberg, Karin& Karni, Arnon. Treatment with Anti-EGF Ab Ameliorates Experimental Autoimmune Encephalomyelitis via Induction of Neurogenesis and Oligodendrogenesis. Multiple Sclerosis International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1046621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046621