Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells
Joint Authors
Brodská, Barbora
Otevřelová, Petra
Holoubek, Aleš
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
While p53-dependent apoptosis is triggered by combination of methyltransferase inhibitor decitabine (DAC) and histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) in leukemic cell line CML-T1, reactive oxygen species (ROS) generation as well as survivin and Bcl-2 deregulation participated in DAC + SAHA-induced apoptosis in p53-deficient HL-60 cell line.
Moreover, decrease of survivin expression level is accompanied by its delocalization from centromere-related position in mitotic cells suggesting that both antiapoptotic and cell cycle regulation roles of survivin are affected by DAC + SAHA action.
Addition of subtoxic concentration of all-trans-retinoic acid (ATRA) increases the efficiency of DAC + SAHA combination on viability, apoptosis induction, and ROS generation in HL-60 cells but has no effect in CML-T1 cell line.
Peripheral blood lymphocytes from healthy donors showed no damage induced by DAC + SAHA + ATRA combination.
Therefore, combination of ATRA with DAC and SAHA represents promising tool for therapy of leukemic disease with nonfunctional p53 signalization.
American Psychological Association (APA)
Brodská, Barbora& Otevřelová, Petra& Holoubek, Aleš. 2014. Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1046952
Modern Language Association (MLA)
Brodská, Barbora…[et al.]. Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1046952
American Medical Association (AMA)
Brodská, Barbora& Otevřelová, Petra& Holoubek, Aleš. Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1046952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046952