Lithium Chloride Suppresses Colorectal Cancer Cell Survival and Proliferation through ROSGSK-3βNF-κB Signaling Pathway
Joint Authors
Li, Huili
Huang, Kun
Liu, Xinghua
Liu, Jinlin
Lu, Xiaoming
Tao, Kaixiong
Wang, Guobin
Wang, Jiliang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Glycogen synthase kinase-3β (GSK-3β), a serine/threonine protein kinase, has been regarded as a potential therapeutic target for multiple human cancers.
In addition, oxidative stress is closely related to all aspects of cancer.
We sought to determine the biological function of lithium, one kind of GSK-3β inhibitors, in the process of reactive oxygen species (ROS) production in colorectal cancer.
In this study, we analyzed the cell apoptosis and proliferation by cell viability, EdU, and flow cytometry assays through administration of LiCl.
We used polymerase chain reaction and Western blotting to establish the effect of GSK-3β inhibition on the nuclear factor-κB (NF-κB) pathway.
Results showed administration of LiCl increased apoptosis and the level of ROS in colorectal cancer cells.
Furthermore, the underlying mechanisms could be mediated by the reduction of NF-κB expression and NF-κB-mediated transcription.
Taken together, our results demonstrated that therapeutic targeting of ROS/GSK-3β/NF-κB pathways may be an effective way for colorectal cancer intervention, although further preclinical and clinical testing are desirable.
American Psychological Association (APA)
Li, Huili& Huang, Kun& Liu, Xinghua& Liu, Jinlin& Lu, Xiaoming& Tao, Kaixiong…[et al.]. 2014. Lithium Chloride Suppresses Colorectal Cancer Cell Survival and Proliferation through ROSGSK-3βNF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1046976
Modern Language Association (MLA)
Li, Huili…[et al.]. Lithium Chloride Suppresses Colorectal Cancer Cell Survival and Proliferation through ROSGSK-3βNF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1046976
American Medical Association (AMA)
Li, Huili& Huang, Kun& Liu, Xinghua& Liu, Jinlin& Lu, Xiaoming& Tao, Kaixiong…[et al.]. Lithium Chloride Suppresses Colorectal Cancer Cell Survival and Proliferation through ROSGSK-3βNF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1046976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046976