Propofol Activation of the Nrf2 Pathway Is Associated with Amelioration of Acute Lung Injury in a Rat Liver Transplantation Model
Joint Authors
Yao, Weifeng
Zhu, Guosong
Chi, Xinjin
Zhang, Ailan
Luo, Gangjian
Xia, Zhengyuan
Hei, Ziqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
This study aimed to investigate whether propofol pretreatment can protect against liver transplantation-induced acute lung injury (ALI) and to explore whether Nrf2 pathway is involved in the protections provided by propofol pretreatment.
Method.
Adult male Sprague-Dawley rats were divided into five groups based on the random number table.
Lung pathology was observed by optical microscopy.
Lung water content was assessed by wet/dry ratio, and PaO2 was detected by blood gas analysis.
The contents of H2O2, MDA, and SOD activity were determined by ELISA method, and the expression of HO-1, NQO1, Keap1, and nuclear Nrf2 was assayed by western blotting.
Results.
Compared with saline-treated model group, both propofol and N-acetylcysteine pretreatment can reduce the acute lung injury caused by orthotopic autologous liver transplantation (OALT), decrease the lung injury scores, lung water content, and H2O2 and MDA levels, and improve the arterial PaO2 and SOD activity.
Furthermore, propofol (but not N-acetylcysteine) pretreatment especially in high dose inhibited the expression of Keap1 and induced translocation of Nrf2 into the nucleus to further upregulate the expression of HO-1 and NQO1 downstream.
Conclusion.
Pretreatment with propofol is associated with attenuation of OALT-induced ALI, and the Nrf2 pathway is involved in the antioxidative processes.
American Psychological Association (APA)
Yao, Weifeng& Luo, Gangjian& Zhu, Guosong& Chi, Xinjin& Zhang, Ailan& Xia, Zhengyuan…[et al.]. 2014. Propofol Activation of the Nrf2 Pathway Is Associated with Amelioration of Acute Lung Injury in a Rat Liver Transplantation Model. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1046982
Modern Language Association (MLA)
Yao, Weifeng…[et al.]. Propofol Activation of the Nrf2 Pathway Is Associated with Amelioration of Acute Lung Injury in a Rat Liver Transplantation Model. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1046982
American Medical Association (AMA)
Yao, Weifeng& Luo, Gangjian& Zhu, Guosong& Chi, Xinjin& Zhang, Ailan& Xia, Zhengyuan…[et al.]. Propofol Activation of the Nrf2 Pathway Is Associated with Amelioration of Acute Lung Injury in a Rat Liver Transplantation Model. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1046982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046982