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BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling
Joint Authors
Zhang, Ming
Yang, Min
Liu, Li-ping
Lau, Wayne Bond
Xin, Man-kun
Su, Li-Xiao
Wang, Jian
Cheng, Shu-Juan
Liu, Jing-Hua
Gao, Hai
Fan, Qian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
The disruption of physiologic vascular smooth muscle cell (VSMC) migration initiates atherosclerosis development.
The biochemical mechanisms leading to dysfunctional VSMC motility remain unknown.
Recently, cytokine BMP-2 has been implicated in various vascular physiologic and pathologic processes.
However, whether BMP-2 has any effect upon VSMC motility, or by what manner, has never been investigated.
Methods.
VSMCs were adenovirally transfected to genetically overexpress BMP-2.
VSMC motility was detected by modified Boyden chamber assay, confocal time-lapse video assay, and a colony wounding assay.
Gene chip array and RT-PCR were employed to identify genes potentially regulated by BMP-2.
Western blot and real-time PCR detected the expression of myosin Va and the phosphorylation of extracellular signal-regulated kinases 1/2 (Erk1/2).
Immunofluorescence analysis revealed myosin Va expression locale.
Intracellular Ca2+ oscillations were recorded.
Results.
VSMC migration was augmented in VSMCs overexpressing BMP-2 in a dose-dependent manner.
siRNA-mediated knockdown of myosin Va inhibited VSMC motility.
Both myosin Va mRNA and protein expression significantly increased after BMP-2 administration and were inhibited by Erk1/2 inhibitor U0126.
BMP-2 induced Ca2+ oscillations, generated largely by a “cytosolic oscillator”.
Conclusion.
BMP-2 significantly increased VSMCs migration and myosin Va expression, via the Erk signaling pathway and intracellular Ca2+ oscillations.
We provide additional insight into the pathophysiology of atherosclerosis, and inhibition of BMP-2-induced myosin Va expression may represent a potential therapeutic strategy.
American Psychological Association (APA)
Zhang, Ming& Yang, Min& Liu, Li-ping& Lau, Wayne Bond& Gao, Hai& Xin, Man-kun…[et al.]. 2014. BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046996
Modern Language Association (MLA)
Zhang, Ming…[et al.]. BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1046996
American Medical Association (AMA)
Zhang, Ming& Yang, Min& Liu, Li-ping& Lau, Wayne Bond& Gao, Hai& Xin, Man-kun…[et al.]. BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046996