Aging Leads to Elevation of O-GlcNAcylation and Disruption of Mitochondrial Homeostasis in Retina
Joint Authors
Zhao, Lin
Feng, Zhihui
Zou, Xuan
Cao, Ke
Xu, Jie
Liu, Jiankang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Retina is particularly susceptible to aging as oxidative damage accumulates within retina, leading to age-related retinal dysfunction or even visual loss.
However, the underlying mechanisms still remain obscure and effective therapeutic strategy is urgently in need.
Here, we quested for the answer particularly focusing on mitochondrial homeostasis and O-GlcNAcylation in rat retina.
By comparing expression of electron transfer chain complexes and key factors in mitochondrial biogenesis and dynamics in retinas of aged and young Sprague-Dawley rats, we found that mitochondrial Complex I, II, IV and V were increased in aged retina with decreased mtTFA and Mfn2.
Also, we noticed that p38 and JNK of MAPK signaling were substantially more activated in aged retina, suggesting stress induction.
In addition, we found that pan-O-GlcNAcylation was remarkably stronger with lower OGA expression in aged retina.
To further elucidate the roles of Mfn2 and O-GlcNAcylation, we employed ARPE-19 cells and found that ATP production, oxygen consumption, and mitochondrial membrane potential were reduced and ROS level was increased by Mfn2 knockdown, while treating with PUGNAc or UDP-GlcNAc heightened oxygen consumption and reduced ROS.
Our results suggest disrupted mitochondrial homeostasis may increase oxidative stress; yet enhanced O-GlcNAcylation might defend against oxidative stress and promote mitochondrial respiration in aged retina.
American Psychological Association (APA)
Zhao, Lin& Feng, Zhihui& Zou, Xuan& Cao, Ke& Xu, Jie& Liu, Jiankang. 2014. Aging Leads to Elevation of O-GlcNAcylation and Disruption of Mitochondrial Homeostasis in Retina. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047027
Modern Language Association (MLA)
Zhao, Lin…[et al.]. Aging Leads to Elevation of O-GlcNAcylation and Disruption of Mitochondrial Homeostasis in Retina. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047027
American Medical Association (AMA)
Zhao, Lin& Feng, Zhihui& Zou, Xuan& Cao, Ke& Xu, Jie& Liu, Jiankang. Aging Leads to Elevation of O-GlcNAcylation and Disruption of Mitochondrial Homeostasis in Retina. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047027