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Response to Rotenone Is Glucose-Sensitive in a Model of Human Acute Lymphoblastic Leukemia: Involvement of Oxidative Stress Mechanism, DJ-1, Parkin, and PINK-1 Proteins
Joint Authors
Mendivil-Perez, Miguel
Velez-Pardo, Carlos
Jimenez-Del-Rio, Marlene
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
To establish the effect of low (11 mM) and high (55 mM) glucose concentrations (G11, G55) on Jurkat cells exposed to rotenone (ROT, a class 5 mitocan).
We demonstrated that ROT induces apoptosis in Jurkat cells cultured in G11 by oxidative stress (OS) mechanism involving the generation of anion superoxide radical ( O 2 ∙ - , 68%)/hydrogen peroxide (H2O2, 54%), activation of NF- κ B (32%), p53 (25%), c-Jun (17%) transcription factors, and caspase-3 (28%), apoptosis-inducing factor (AIF, 36%) nuclei translocation, c-Jun N-terminal kinase (JNK) activation, and loss of mitochondria transmembrane potential ( Δ Ψ m , 62%) leading to nuclei fragmentation (~10% and ~40% stage I-II fragmented nuclei, resp.).
ROT induces massive cytoplasmic aggregates of DJ-1 (93%), and upregulation of Parkin compared to untreated cells, but no effect on PINK-1 protein was observed.
Cell death marker detection and DJ-1 and Parkin expression were significantly reduced when cells were cultured in G55 plus ROT.
Remarkably, metformin sensitized Jurkat cells against ROT in G55.
Our results indicate that a high-glucose milieu promotes resistance against ROT/H2O2-induced apoptosis in Jurkat cells.
Our data suggest that combined therapy by using mitochondria-targeted damaging compounds and regulation of glucose (e.g., metformin) can efficiently terminate leukemia cells via apoptosis in hyperglycemic conditions.
American Psychological Association (APA)
Mendivil-Perez, Miguel& Jimenez-Del-Rio, Marlene& Velez-Pardo, Carlos. 2014. Response to Rotenone Is Glucose-Sensitive in a Model of Human Acute Lymphoblastic Leukemia: Involvement of Oxidative Stress Mechanism, DJ-1, Parkin, and PINK-1 Proteins. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047036
Modern Language Association (MLA)
Mendivil-Perez, Miguel…[et al.]. Response to Rotenone Is Glucose-Sensitive in a Model of Human Acute Lymphoblastic Leukemia: Involvement of Oxidative Stress Mechanism, DJ-1, Parkin, and PINK-1 Proteins. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1047036
American Medical Association (AMA)
Mendivil-Perez, Miguel& Jimenez-Del-Rio, Marlene& Velez-Pardo, Carlos. Response to Rotenone Is Glucose-Sensitive in a Model of Human Acute Lymphoblastic Leukemia: Involvement of Oxidative Stress Mechanism, DJ-1, Parkin, and PINK-1 Proteins. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047036