Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract
Joint Authors
Reddy, Geereddy Bhanuprakash
Patil, Madhoosudan A.
Suryanarayana, Palla
Putcha, Uday Kumar
Srinivas, Myadara
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Type 2 diabetes (T2D) generally follows prediabetes (PD) conditions such as impaired fasting glucose (IFG) and/or impaired glucose tolerance (IGT).
Although studies reported an association of IGT or IFG with cataract, the experimental basis for PD associated cataract is not known.
Hence, we evaluated neonatal streptozotocin (nSTZ) induced rat model to study PD associated cataractogenesis by injecting STZ to two-day old rats.
While majority (70%) of nSTZ injected pups developed IGT (nSTZ-PD) by two months but not cataract even after seven months, remaining (30%) nSTZ rats developed hyperglycemia (nSTZ-D) by two months and mature cataract by seven months.
Lens biochemical analysis indicated increased oxidative stress as indicated by increased SOD activity, lipid peroxidation, and protein carbonyl levels in nSTZ-D cataractous lens.
There was also increased polyol pathway as assessed by aldose reductase activity and sorbitol levels.
Though nSTZ-PD animals have not shown any signs of lenticular opacity, insolubilization of proteins along with enhanced polyol pathway was observed in the lens.
Further there was increased oxidative stress in lens of IGT animals.
These results suggest that oxidative stress along with increased polyol pathway might play a role in IGT-associated lens abnormalities.
In conclusion, nSTZ-PD rat model could aid to investigate IGT-associated lens abnormalities.
American Psychological Association (APA)
Patil, Madhoosudan A.& Suryanarayana, Palla& Putcha, Uday Kumar& Srinivas, Myadara& Reddy, Geereddy Bhanuprakash. 2014. Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047039
Modern Language Association (MLA)
Patil, Madhoosudan A.…[et al.]. Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047039
American Medical Association (AMA)
Patil, Madhoosudan A.& Suryanarayana, Palla& Putcha, Uday Kumar& Srinivas, Myadara& Reddy, Geereddy Bhanuprakash. Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047039