Insulin Regulates Glucose Consumption and Lactate Production through Reactive Oxygen Species and Pyruvate Kinase M2
Joint Authors
Li, Qi
Liu, Xue
Yin, Yu
Zheng, Ji-Tai
Jiang, Cheng-Fei
Wang, Jing
Shen, Hua
Li, Chong-Yong
Wang, Min
Liu, Ling-Zhi
Jiang, Bing-Hua
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Although insulin is known to regulate glucose metabolism and closely associate with liver cancer, the molecular mechanisms still remain to be elucidated.
In this study, we attempt to understand the mechanism of insulin in promotion of liver cancer metabolism.
We found that insulin increased pyruvate kinase M2 (PKM2) expression through reactive oxygen species (ROS) for regulating glucose consumption and lactate production, key process of glycolysis in hepatocellular carcinoma HepG2 and Bel7402 cells.
Interestingly, insulin-induced ROS was found responsible for the suppression of miR-145 and miR-128, and forced expression of either miR-145 or miR-128 was sufficient to abolish insulin-induced PKM2 expression.
Furthermore, the knockdown of PKM2 expression also inhibited cancer cell growth and insulin-induced glucose consumption and lactate production, suggesting that PKM2 is a functional downstream effecter of insulin.
Taken together, this study would provide a new insight into the mechanism of insulin-induced glycolysis.
American Psychological Association (APA)
Li, Qi& Liu, Xue& Yin, Yu& Zheng, Ji-Tai& Jiang, Cheng-Fei& Wang, Jing…[et al.]. 2014. Insulin Regulates Glucose Consumption and Lactate Production through Reactive Oxygen Species and Pyruvate Kinase M2. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047049
Modern Language Association (MLA)
Li, Qi…[et al.]. Insulin Regulates Glucose Consumption and Lactate Production through Reactive Oxygen Species and Pyruvate Kinase M2. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047049
American Medical Association (AMA)
Li, Qi& Liu, Xue& Yin, Yu& Zheng, Ji-Tai& Jiang, Cheng-Fei& Wang, Jing…[et al.]. Insulin Regulates Glucose Consumption and Lactate Production through Reactive Oxygen Species and Pyruvate Kinase M2. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047049