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Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Gwee Sian Khee, Sharon
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Emerging evidences highlight the implication of microRNAs as a posttranscriptional regulator in aging.
Several senescence-associated microRNAs (SA-miRNAs) are found to be differentially expressed during cellular senescence.
However, the role of dietary compounds on SA-miRNAs remains elusive.
This study aimed to elucidate the modulatory role of tocotrienol-rich fraction (TRF) on SA-miRNAs (miR-20a, miR-24, miR-34a, miR-106a, and miR-449a) and established target genes of miR-34a (CCND1, CDK4, and SIRT1) during replicative senescence of human diploid fibroblasts (HDFs).
Primary cultures of HDFs at young and senescent were incubated with TRF at 0.5 mg/mL.
Taqman microRNA assay showed significant upregulation of miR-24 and miR-34a and downregulation of miR-20a and miR-449a in senescent HDFs (P<0.05).
TRF reduced miR-34a expression in senescent HDFs and increased miR-20a expression in young HDFs and increased miR-449a expression in both young and senescent HDFs.
Our results also demonstrated that ectopic expression of miR-34a reduced the expression of CDK4 significantly (P<0.05).
TRF inhibited miR-34a expression thus relieved its inhibition on CDK4 gene expression.
No significant change was observed on the expression of CCND1, SIRT1, and miR-34a upstream transcriptional regulator, TP53.
In conclusion tocotrienol-rich fraction prevented cellular senescence of human diploid fibroblasts via modulation of SA-miRNAs and target genes expression.
American Psychological Association (APA)
Gwee Sian Khee, Sharon& Mohd Yusof, Yasmin Anum& Makpol, Suzana. 2014. Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047098
Modern Language Association (MLA)
Gwee Sian Khee, Sharon…[et al.]. Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047098
American Medical Association (AMA)
Gwee Sian Khee, Sharon& Mohd Yusof, Yasmin Anum& Makpol, Suzana. Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047098