PKCδ Promotes High Glucose Induced Renal Tubular Oxidative Damage via Regulating Activation and Translocation of p66Shc
Joint Authors
Yang, Shikun
Song, Panai
Xiao, Li
Xu, Xiaoxuan
Tang, Chengyuan
Yang, Yuyan
Ma, Mingming
Zhu, Jiefu
Liu, Fuyou
Sun, Lin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Diabetic kidney disease (DKD) is a leading cause of end-stage renal disease (ESRD).
Renal tubular injury by overproduction of ROS in mitochondria plays a critical role in the pathogenesis of DKD.
Evidences have shown that p66Shc was involved in renal tubular injury via mitochondrial-dependent ROS production pathway, but little is known about the upstream signaling of p66Shc that leads to tubular oxidative damage under high glucose conditions.
In this study, an increased PKCδ and p66Shc activation and ROS production in renal tissues of patients with diabetic nephropathy were seen and further analysis revealed a positive correlation between the tubulointerstitial damage and p-PKCδ, p-p66Shc, and ROS production.
In vitro, we investigated the phosphorylation and activation of p66Shc and PKCδ during treatment of HK-2 cells with high glucose (HG).
Results showed that the activation of p66Shc and PKCδ was increased in a dose- and time-dependent manner, and this effect was suppressed by Rottlerin, a pharmacologic inhibitor of PKCδ.
Moreover, PKCδ siRNA partially blocked HG-induced p66Shc phosphorylation, translocation, and ROS production in HK-2 cells.
Taken together, these data suggest that activation of PKCδ promotes tubular cell injury through regulating p66Shc phosphorylation and mitochondrial translocation in HG ambient.
American Psychological Association (APA)
Song, Panai& Yang, Shikun& Xiao, Li& Xu, Xiaoxuan& Tang, Chengyuan& Yang, Yuyan…[et al.]. 2014. PKCδ Promotes High Glucose Induced Renal Tubular Oxidative Damage via Regulating Activation and Translocation of p66Shc. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047107
Modern Language Association (MLA)
Song, Panai…[et al.]. PKCδ Promotes High Glucose Induced Renal Tubular Oxidative Damage via Regulating Activation and Translocation of p66Shc. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047107
American Medical Association (AMA)
Song, Panai& Yang, Shikun& Xiao, Li& Xu, Xiaoxuan& Tang, Chengyuan& Yang, Yuyan…[et al.]. PKCδ Promotes High Glucose Induced Renal Tubular Oxidative Damage via Regulating Activation and Translocation of p66Shc. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047107
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047107