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Dual Stimulus-Dependent Effect of Oenothera paradoxa Extract on the Respiratory Burst in Human Leukocytes: Suppressing for Escherichia coli and Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine
Joint Authors
Burzynska-Pedziwiatr, Izabela
Bukowiecka-Matusiak, Malgorzata
Wojcik, Marzena
Machala, Waldemar
Bienkiewicz, Malgorzata
Spolnik, Grzegorz
Danikiewicz, Witold
Wozniak, Lucyna Alicja
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Although a growing body of evidence suggests that plant polyphenols can modulate human immune responses, their simultaneous action on monocyte and neutrophil oxidative burst is currently poorly understood.
Based on the hypothesis that various polyphenols contained in plant extracts might affect the oxidative burst of phagocytes, we evaluated the effects of ethanolic O.
paradoxa extract polyphenols on monocyte and neutrophil oxidative burst in vitro activated by different stimuli, including opsonized bacteria E.
coli, phorbol 12-myristate 13-acetate (PMA), and formyl-methionyl-leucyl-phenylalanine (fMLP).
Samples were analyzed by the dihydrorhodamine flow cytometry assay.
Our results showed that the extract repressed significantly and dose-dependently reactive oxygen species production in both cell types stimulated with E.
coli and PMA (P < 0.05) and its inhibitory efficiency was stimulus- and cell-type-dependent.
Interestingly, there was significant stimulatory effect of the extract on bursting phagocytes induced by fMLP (P < 0.05).
Additionally, several flavonoids and phenolic compounds as well as penta-galloyl-β-(D)-glucose (PGG), the representative of hydrolyzable tannins, were identified in the 60% extract by high-performance liquid chromatography (HPLC) coupled to electrospray ionization in negative ion mode.
In summary, the ethanolic O.
paradoxa extract, rich in flavonoids and phenolic compounds, exhibits dual stimulus-dependent effect on the respiratory burst in human leukocytes; hence, it might affect immune responses in humans.
American Psychological Association (APA)
Burzynska-Pedziwiatr, Izabela& Bukowiecka-Matusiak, Malgorzata& Wojcik, Marzena& Machala, Waldemar& Bienkiewicz, Malgorzata& Spolnik, Grzegorz…[et al.]. 2014. Dual Stimulus-Dependent Effect of Oenothera paradoxa Extract on the Respiratory Burst in Human Leukocytes: Suppressing for Escherichia coli and Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047118
Modern Language Association (MLA)
Burzynska-Pedziwiatr, Izabela…[et al.]. Dual Stimulus-Dependent Effect of Oenothera paradoxa Extract on the Respiratory Burst in Human Leukocytes: Suppressing for Escherichia coli and Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1047118
American Medical Association (AMA)
Burzynska-Pedziwiatr, Izabela& Bukowiecka-Matusiak, Malgorzata& Wojcik, Marzena& Machala, Waldemar& Bienkiewicz, Malgorzata& Spolnik, Grzegorz…[et al.]. Dual Stimulus-Dependent Effect of Oenothera paradoxa Extract on the Respiratory Burst in Human Leukocytes: Suppressing for Escherichia coli and Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047118