Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
Joint Authors
Lavazza, Antonio
Sberveglieri, Giorgio
Stacchiotti, Alessandra
Rovetta, Francesca
Ferroni, Matteo
Corsetti, Giovanni
Aleo, Maria Francesca
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia.
Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle.
To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt.
Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs.
Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm.
Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h.
Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes.
Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo.
American Psychological Association (APA)
Stacchiotti, Alessandra& Rovetta, Francesca& Ferroni, Matteo& Corsetti, Giovanni& Lavazza, Antonio& Sberveglieri, Giorgio…[et al.]. 2014. Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047148
Modern Language Association (MLA)
Stacchiotti, Alessandra…[et al.]. Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047148
American Medical Association (AMA)
Stacchiotti, Alessandra& Rovetta, Francesca& Ferroni, Matteo& Corsetti, Giovanni& Lavazza, Antonio& Sberveglieri, Giorgio…[et al.]. Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047148