![](/images/graphics-bg.png)
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPKNF-κB Signaling
Joint Authors
Yuan, Shi-fang
Lv, Yong-gang
Chen, Jiang-hao
Wang, Lei
Yu, Feng
Zhao, Ya-li
Wang, Ling
Yi, Jun
Bian, Jie-fang
Wang, Ting
Liu, Ning-ning
Zhu, Xiao-shan
Yun, Jun
Ling, Rui
Li, Jun-tang
Wang, Hui
Li, Nan-lin
Wang, Ling
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Volatile anesthetic isoflurane (ISO) has immunomodulatory effects.
The fungal component zymosan (ZY) induces inflammation through toll-like receptor 2 or dectin-1 signaling.
We investigated the molecular actions of subanesthetic (0.7%) ISO against ZY-induced inflammatory activation in murine Kupffer cells (KCs), which are known as the resident macrophages within the liver.
We observed that ISO reduced ZY-induced cyclooxygenase 2 upregulation and prostaglandin E2 release, as determined by western blot and radioimmunoassay, respectively.
ISO also reduced the production of tumor necrosis factor-α, interleukin-1β, IL-6, high-mobility group box-1, macrophage inflammatory protein-1α, macrophage inflammatory protein-2, and monocyte chemoattractant protein-1 as assessed by enzyme-linked immunosorbent assays.
ISO blocked the ZY-induced nuclear translocation and DNA-binding activity of nuclear factor- (NF)-κB p65.
Moreover, ISO attenuated ZY-induced p38 mitogen-activated protein kinase (MAPK) activation partly by scavenging reactive oxygen species (ROS); the interregulation that ROS activated p38 MAPK followed by NF-κB activation was crucial for the ZY-induced inflammatory responses in KCs.
An in vivo study by peritoneal injection of ZY into BALB/C mice confirmed the anti-inflammatory properties of 0.7% ISO against ZY in KCs.
These results suggest that ISO ameliorates ZY-induced inflammatory responses in murine KCs by inhibiting the interconnected ROS/p38 MAPK/NF-κB signaling pathways.
American Psychological Association (APA)
Wang, Hui& Wang, Lei& Li, Nan-lin& Li, Jun-tang& Yu, Feng& Zhao, Ya-li…[et al.]. 2014. Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPKNF-κB Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047149
Modern Language Association (MLA)
Wang, Hui…[et al.]. Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPKNF-κB Signaling. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1047149
American Medical Association (AMA)
Wang, Hui& Wang, Lei& Li, Nan-lin& Li, Jun-tang& Yu, Feng& Zhao, Ya-li…[et al.]. Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPKNF-κB Signaling. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047149
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047149