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Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF- κ B Signaling Pathway
Joint Authors
Hseu, You Cheng
Yang, Hsin-Ling
Hsu, Li-Sung
Huang, Pei-Jane
Liu, Yi-Ru
Lu, Te-Ling
Chia, Yi-Chen
Takajo, Tokuko
Kazunori, Anzai
Kumar, K. J. Senthil
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Toona sinensis is one of the most popular vegetarian cuisines in Taiwan and it has been shown to possess antioxidant, antiangiogenic, and anticancer properties.
In this study, we investigated the antiatherosclerotic potential of aqueous leaf extracts from Toona sinensis (TS; 25–100 μ g/mL) and its major bioactive compound, gallic acid (GA; 5 μ g/mL), in LPS-treated rat aortic smooth muscle (A7r5) cells.
We found that pretreatment with noncytotoxic concentrations of TS and GA significantly inhibited inflammatory NO and PGE 2 production by downregulating their precursors, iNOS and COX-2, respectively, in LPS-treated A7r5 cells.
Furthermore, TS and GA inhibited LPS-induced intracellular ROS and their corresponding mediator, p4 7 phox .
Notably, TS and GA pretreatment significantly inhibited LPS-induced migration in transwell assays.
Gelatin zymography and western blotting demonstrated that treatment with TS and GA suppressed the activity or expression of MMP-9, MMP-2, and t-PA.
Additionally, TS and GA significantly inhibited LPS-induced VEGF, PDGF, and VCAM-1 expression.
Further investigation revealed that the inhibition of iNOS/COX-2, MMPs, growth factors, and adhesion molecules was associated with the suppression of NF-κB activation and MAPK (ERK1/2, JNK1/2, and p38) phosphorylation.
Thus, Toona sinensis may be useful for the prevention of atherosclerosis.
American Psychological Association (APA)
Yang, Hsin-Ling& Huang, Pei-Jane& Liu, Yi-Ru& Kumar, K. J. Senthil& Hsu, Li-Sung& Lu, Te-Ling…[et al.]. 2014. Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF- κ B Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047158
Modern Language Association (MLA)
Yang, Hsin-Ling…[et al.]. Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF- κ B Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1047158
American Medical Association (AMA)
Yang, Hsin-Ling& Huang, Pei-Jane& Liu, Yi-Ru& Kumar, K. J. Senthil& Hsu, Li-Sung& Lu, Te-Ling…[et al.]. Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF- κ B Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047158
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047158