Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression
Joint Authors
Bányai, Emese
Balogh, Enikő
Fagyas, Miklós
Hendrik, Zoltán
Király, Gábor
Nagy, Gábor
Tánczos, Bence
Pócsi, István
Balla, György
Balla, József
Bánfalvi, Gáspár
Jeney, Viktória
Arosio, Paolo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Podocytes are highly specialized, arborized epithelial cells covering the outer surface of the glomerular tuft in the kidney.
Terminally differentiated podocytes are unable to go through cell division and hereby they are lacking a key property for regeneration after a toxic injury.
Podocytes are long-lived cells but, to date, little is known about the mechanisms that support their stress resistance.
Our aim was to investigate whether the well-known morphological changes during podocyte differentiation are accompanied by changes in oxidative resistance in a manner that could support their long-term survival.
We used a conditionally immortalized human podocyte cell line to study the morphological and functional changes during differentiation.
We followed the differentiation process for 14 days by time-lapse microscopy.
During this period nondifferentiated podocytes gradually transformed into large, nonproliferating, frequently multinucleated cells, with enlarged nuclei and opened chromatin structure.
We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges.
Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression.
Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.
American Psychological Association (APA)
Bányai, Emese& Balogh, Enikő& Fagyas, Miklós& Arosio, Paolo& Hendrik, Zoltán& Király, Gábor…[et al.]. 2014. Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047173
Modern Language Association (MLA)
Bányai, Emese…[et al.]. Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047173
American Medical Association (AMA)
Bányai, Emese& Balogh, Enikő& Fagyas, Miklós& Arosio, Paolo& Hendrik, Zoltán& Király, Gábor…[et al.]. Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047173