Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease
Joint Authors
Sen, Oishimaya
Pramanik, Vishmadeb
Roy Mukherjee, Tapasi
Banerjee, Kalpita
Munshi, Soumyabrata
Chakrabarti, Sasanka
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
The cytotoxic effects of dopamine (DA) on several catecholaminergic cell lines involve DA oxidation products like reactive oxygen species (ROS) and toxic quinones and have implications in the pathogenesis of sporadic Parkinson's disease (PD).
However, many molecular details are yet to be elucidated, and the possible nonoxidative mechanism of dopamine cytotoxicity has not been studied in great detail.
Results.
Cultured SH-SY5Y cells treated with DA (up to 400 μM) or lactacystin (5 μM) or DA (400 μM) plus N-acetylcysteine (NAC, 2.5 mM) for 24 h are processed accordingly to observe the cell viability, mitochondrial dysfunctions, oxidative stress parameters, proteasomal activity, expression of alpha-synuclein gene, and intracellular accumulation of the protein.
DA causes mitochondrial dysfunction and extensive loss of cell viability partially inhibited by NAC, potent inhibition of proteasomal activity marginally prevented by NAC, and overexpression with accumulation of intracellular alpha-synuclein partially preventable by NAC.
Under similar conditions of incubation, NAC completely prevents enhanced production of ROS and increased formation of quinoprotein adducts in DA-treated SH-SY5Y cells.
Separately, proteasomal inhibitor lactacystin causes accumulation of alpha-synuclein as well as mitochondrial dysfunction and cell death.
Conclusions.
DA cytotoxicity includes both oxidative and nonoxidative modes and may involve overexpression and accumulation of alpha-synuclein as well as proteasomal inhibition.
American Psychological Association (APA)
Banerjee, Kalpita& Munshi, Soumyabrata& Sen, Oishimaya& Pramanik, Vishmadeb& Roy Mukherjee, Tapasi& Chakrabarti, Sasanka. 2014. Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease. Parkinson’s Disease،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047229
Modern Language Association (MLA)
Banerjee, Kalpita…[et al.]. Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease. Parkinson’s Disease No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047229
American Medical Association (AMA)
Banerjee, Kalpita& Munshi, Soumyabrata& Sen, Oishimaya& Pramanik, Vishmadeb& Roy Mukherjee, Tapasi& Chakrabarti, Sasanka. Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease. Parkinson’s Disease. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047229