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Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry
Joint Authors
Katsura, Shogo
Okumura, Tomoko
Ito, Ryo
Sugawara, Akira
Yokoyama, Atsushi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Posttranslational modification (PTM) of proteins is critical for various cellular processes.
However, there are few studies examining PTMs in specific proteins using unbiased approaches.
Here we report the attempt to identify the PTMs in peroxisome proliferator-activated receptor γ (PPARγ) proteins using our previously established PTM analysis system.
In this study, we identified several PTMs in exogenously expressed PPARγ2 proteins from 293T cells as well as endogenous PPARγ1 proteins from a Caco-2 colon cancer cell line.
The identified PTMs include phosphorylation of serine 112 and serine 81 in PPARγ2 and PPARγ1, respectively, both of which are well-known mitogen-activated protein kinase- (MAP kinase-) mediated PTMs in PPARγ proteins, thus confirming our experimental approach.
Furthermore, previously unknown PTMs were also identified, demonstrating that this method can be applied to find previously unidentified PTMs in PPARγ proteins and other proteins including nuclear receptors.
American Psychological Association (APA)
Katsura, Shogo& Okumura, Tomoko& Ito, Ryo& Sugawara, Akira& Yokoyama, Atsushi. 2014. Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry. PPAR Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047278
Modern Language Association (MLA)
Katsura, Shogo…[et al.]. Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry. PPAR Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1047278
American Medical Association (AMA)
Katsura, Shogo& Okumura, Tomoko& Ito, Ryo& Sugawara, Akira& Yokoyama, Atsushi. Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry. PPAR Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047278
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047278