Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model
Joint Authors
Tsai, Tzu-Hsien
Sun, Cheuk-Kwan
Zhen, Yen-Yi
Lu, Hung-I
Sung, Pei-Hsun
Chang, Li-Teh
Sheu, Jiunn-Jye
Chen, Yung-Lung
Chua, Sarah
Chang, Hsueh-Wen
Chen, Yi-Ling
Lee, Fan-Yen
Yip, Hon-Kan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-18
Country of Publication
Egypt
No. of Pages
19
Abstract EN
We tested the hypothesis that Lipofectamine siRNA delivery to deplete transient receptor potential cation channel (TRPC) 1 protein expression can suppress hypoxia-induced pulmonary arterial hypertension (PAH) in mice.
Adult male C57BL/6 mice were equally divided into group 1 (normal controls), group 2 (hypoxia), and group 3 (hypoxia + siRNA TRPC1).
By day 28, right ventricular systolic pressure (RVSP), number of muscularized arteries, right ventricle (RV), and lung weights were increased in group 2 than in group 1 and reduced in group 3 compared with group 2.
Pulmonary crowded score showed similar pattern, whereas number of alveolar sacs exhibited an opposite pattern compared to that of RVSP in all groups.
Protein expressions of TRPCs, HIF-1α, Ku-70, apoptosis, and fibrosis and pulmonary mRNA expressions of inflammatory markers were similar pattern, whereas protein expressions of antifibrosis and VEGF were opposite to the pattern of RVSP.
Cellular markers of pulmonary DNA damage, repair, and smooth muscle proliferation exhibited a pattern similar to that of RVSP.
The mRNA expressions of proapoptotic and hypertrophy biomarkers displayed a similar pattern, whereas sarcomere length showed an opposite pattern compared to that of RVSP in all groups.
Lipofectamine siRNA delivery effectively reduced TRPC1 expression, thereby attenuating PAH-associated RV and pulmonary arteriolar remodeling.
American Psychological Association (APA)
Sun, Cheuk-Kwan& Zhen, Yen-Yi& Lu, Hung-I& Sung, Pei-Hsun& Chang, Li-Teh& Tsai, Tzu-Hsien…[et al.]. 2014. Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model. Stem Cells International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1047506
Modern Language Association (MLA)
Sun, Cheuk-Kwan…[et al.]. Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model. Stem Cells International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-1047506
American Medical Association (AMA)
Sun, Cheuk-Kwan& Zhen, Yen-Yi& Lu, Hung-I& Sung, Pei-Hsun& Chang, Li-Teh& Tsai, Tzu-Hsien…[et al.]. Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1047506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047506