Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs

Joint Authors

Soares, Milena Botelho
Penha, Euler Moraes
Meira, Cássio Santana
Guimarães, Elisalva Teixeira
Mendonça, Marcus Vinícius Pinheiro
Gravely, Faye Alice
Pinheiro, Cláudia Maria Bahia
Pinheiro, Taiana Maria Bahia
Barrouin-Melo, Stella Maria
Ribeiro-dos-Santos, Ricardo

Source

Stem Cells International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-02-25

Country of Publication

Egypt

No. of Pages

8

Abstract EN

The use of stem cells in injury repair has been extensively investigated.

Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries.

MSC were cultured in vitro, and proliferation rate and cell viability were evaluated.

Cell suspensions were prepared and surgically administered into the spinal cord.

The animals were clinically evaluated and examined by nuclear magnetic resonance.

Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses.

Each dog demonstrated an improvement in these gains over time.

Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs.

By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs.

However, no clinical gain was associated with alterations in magnetic resonance imaging.

Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury.

American Psychological Association (APA)

Penha, Euler Moraes& Meira, Cássio Santana& Guimarães, Elisalva Teixeira& Mendonça, Marcus Vinícius Pinheiro& Gravely, Faye Alice& Pinheiro, Cláudia Maria Bahia…[et al.]. 2014. Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs. Stem Cells International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047517

Modern Language Association (MLA)

Penha, Euler Moraes…[et al.]. Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs. Stem Cells International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1047517

American Medical Association (AMA)

Penha, Euler Moraes& Meira, Cássio Santana& Guimarães, Elisalva Teixeira& Mendonça, Marcus Vinícius Pinheiro& Gravely, Faye Alice& Pinheiro, Cláudia Maria Bahia…[et al.]. Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047517

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1047517