Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth
Joint Authors
Karaöz, Erdal
Aksoy, Ayca
Duruksu, Gokhan
Gacar, Gulcin
Akpinar, Gurler
Kasap, Murat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-13
Country of Publication
Egypt
No. of Pages
19
Abstract EN
The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use.
The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches.
The cells were isolated from a natal (NDPSCs), an exfoliated deciduous (stem cells from human exfoliated deciduous (SHED)), and an impacted third molar (DPSCs) tooth of three different donors.
All three stem cells displayed similar features related to morphology, proliferation rates, expression of various cell surface markers, and differentiation potentials into adipocytes, osteocytes, and chondrocytes.
Furthermore, using 2DE approach coupled with MALDI-TOF/TOF, we have generated a common 2DE profile for all three stem cells.
We found that 62.3 ± 7 % of the protein spots were conserved among the three mesenchymal stem cell lines.
Sixty-one of these conserved spots were identified by MALDI-TOF/TOF analysis.
Classification of the identified proteins based on biological function revealed that structurally important proteins and proteins that are involved in protein folding machinery are predominantly expressed by all three stem cell lines.
Some of these proteins may hold importance in understanding specific properties of human dental pulp derived mesenchymal stem cells.
American Psychological Association (APA)
Akpinar, Gurler& Kasap, Murat& Aksoy, Ayca& Duruksu, Gokhan& Gacar, Gulcin& Karaöz, Erdal. 2014. Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth. Stem Cells International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1047519
Modern Language Association (MLA)
Akpinar, Gurler…[et al.]. Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth. Stem Cells International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-1047519
American Medical Association (AMA)
Akpinar, Gurler& Kasap, Murat& Aksoy, Ayca& Duruksu, Gokhan& Gacar, Gulcin& Karaöz, Erdal. Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-1047519
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047519