Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro
Joint Authors
Yamamoto, Maiko
Nakata, Hidemi
Hao, Jia
Chou, Joshua
Kasugai, Shohei
Kuroda, Shinji
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-15
Country of Publication
Egypt
No. of Pages
17
Abstract EN
Adipose tissue-derived stromal cells, termed ASCs, play an important role in regenerative applications.
They resemble mesenchymal stem cells owing to their inexhaustibility, general differentiation potential, and plasticity and display a series of cell-specific and cluster-of-differentiation (CD) marker profiles similar to those of other somatic stem cells.
Variations in phenotypes or differentiation are intimately associated with CD markers.
The purpose of our study was to exhibit distinct populations of ASCs with differing characteristics for osteogenic differentiation.
The primary cell batch of murine-derived ASCs was extracted from subcutaneous adipose tissue and the cells were sorted for the expression of the surface protein molecules CD90 and CD105 using flow cytometry.
Each cell population sorted for CD90 and CD105 was analyzed for osteogenic potency after cell culture.
The results suggested that ASCs exhibit distinct populations with differing characteristics for osteogenic differentiation: unsorted ASCs stimulated comparable mineralized nodule formation as bone marrow stromal cells (BMSCs) in osteogenic medium and viral transfection for BMP2 accelerated the formation of mineralized nodules in CD90 and/or CD105 positive ASCs with observation of decrease in CD105 expression after 14 days.
Future studies assessing different immunophenotypes of ASCs should be undertaken to develop cell-based tissue engineering.
American Psychological Association (APA)
Yamamoto, Maiko& Nakata, Hidemi& Hao, Jia& Chou, Joshua& Kasugai, Shohei& Kuroda, Shinji. 2014. Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro. Stem Cells International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1047525
Modern Language Association (MLA)
Yamamoto, Maiko…[et al.]. Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro. Stem Cells International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-1047525
American Medical Association (AMA)
Yamamoto, Maiko& Nakata, Hidemi& Hao, Jia& Chou, Joshua& Kasugai, Shohei& Kuroda, Shinji. Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1047525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047525