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Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study
Joint Authors
Ghorai, Atanu
Bhattacharyya, Nitai P.
Sarma, Asitikantha
Ghosh, Utpal
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cancer treatment with high LET heavy ion beam, especially, carbon ion beam (12C), is becoming very popular over conventional radiotherapy like low LET gamma or X-ray.
Combination of Poly(ADP-ribose) polymerase (PARP) inhibitor with xenotoxic drugs or conventional radiation (gamma or X-ray) is the newer approach for cancer therapy.
The aim of our study was to compare the radiosensitivity and induction of apoptosis by high LET 12C and low LET gamma radiation in HeLa and PARP-1 knocked down cells.
We did comet assay to detect DNA breaks, clonogenic survival assay, and cell cycle analysis to measure recovery after DNA damage.
We measured apoptotic parameters like nuclear fragmentation and caspase-3 activation.
DNA damage, cell killing, and induction of apoptosis were significantly higher for 12C than gamma radiation in HeLa.
Cell killing and apoptosis were further elevated upon knocking down of PARP-1.
Both 12C and gamma induced G2/M arrest although the 12C had greater effect.
Unlike the gamma, 12C irradiation affects DNA replication as detected by S-phase delay in cell cycle analysis.
So, we conclude that high LET 12C has greater potential over low LET gamma radiation in killing cells and radiosensitization upon PARP-1 inhibition was several folds greater for 12C than gamma.
American Psychological Association (APA)
Ghorai, Atanu& Bhattacharyya, Nitai P.& Sarma, Asitikantha& Ghosh, Utpal. 2014. Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study. Scientifica،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047565
Modern Language Association (MLA)
Ghorai, Atanu…[et al.]. Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study. Scientifica No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047565
American Medical Association (AMA)
Ghorai, Atanu& Bhattacharyya, Nitai P.& Sarma, Asitikantha& Ghosh, Utpal. Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study. Scientifica. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047565