Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran
Joint Authors
Soleimanpour, Saman
Hamedi Asl, Daryoush
Tadayon, Keyvan
Farazi, Ali Asghar
Keshavarz, Rouhollah
Soleymani, Kioomars
Seddighinia, Fereshteh Sadat
Mosavari, Nader
Source
Tuberculosis Research and Treatment
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Human tuberculosis caused by Mycobacterium tuberculosis (Mtb) remains a significant disease in many countries.
According to Iran’s borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epidemiology of tuberculosis and survey genetic diversity of Mtb strains in Markazi Province in center of Iran.
In this experimental study, 75 sputum specimens and one gastric lavage from all smear-positive TB patients admitted to the public hospitals across the Markazi Province were cultured on specific mycobacterial culture media.
Genomic DNA was digested by PvuII and transferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS and DR probes.
Genotyping of the isolates by PGRS-RFLP and DR-RFLP displayed a wide range of genetic diversity as 25 and 26 genotypes were identified, respectively.
Generally speaking, despite the relatively limited number of isolates in the study, high age of patients and also large heterogeneity found in the setting are both in opposition to active circulation of Mtb strains between patients under study either Iranian or Afghan nationals.
Thus, it seems that reactivation of latent infection has had the main role in the spread of tuberculosis.
American Psychological Association (APA)
Soleimanpour, Saman& Hamedi Asl, Daryoush& Tadayon, Keyvan& Farazi, Ali Asghar& Keshavarz, Rouhollah& Soleymani, Kioomars…[et al.]. 2014. Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberculosis Research and Treatment،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1048055
Modern Language Association (MLA)
Soleimanpour, Saman…[et al.]. Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberculosis Research and Treatment No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1048055
American Medical Association (AMA)
Soleimanpour, Saman& Hamedi Asl, Daryoush& Tadayon, Keyvan& Farazi, Ali Asghar& Keshavarz, Rouhollah& Soleymani, Kioomars…[et al.]. Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberculosis Research and Treatment. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1048055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048055