Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
Joint Authors
Abdul Razak, Fathilah
Majid, Mohd Zabidi
Mohamad Zaini, Zuraiza
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation.
This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death.
The concentration producing 50% cell inhibition (IC50) was determined and the activity was examined under an inverted microscope.
Immunohistochemistry fluorescent staining method (TUNEL) was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification.
Compared to Azadirachta sp.
and Typhonium sp., the antiproliferative activity of Brucea sp.
extract was the most potent on both KB and ORL-48 cells with IC50 of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively.
Signs of cell attrition were observed 24 hr after treatment.
Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts.
DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract.
It is thus concluded that Brucea sp.
extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis.
American Psychological Association (APA)
Majid, Mohd Zabidi& Mohamad Zaini, Zuraiza& Abdul Razak, Fathilah. 2014. Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048374
Modern Language Association (MLA)
Majid, Mohd Zabidi…[et al.]. Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1048374
American Medical Association (AMA)
Majid, Mohd Zabidi& Mohamad Zaini, Zuraiza& Abdul Razak, Fathilah. Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048374