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Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol
Joint Authors
Bhauso, Tengale Dipak
Radhakrishnan, Thankappan
Kumar, Abhay
Mishra, Gyan Prakash
Dobaria, Jentilal Ramjibhai
Patel, Kirankumar
Rajam, Manchikatla Venkat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
In the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L.) crop yield.
In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms.
Mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many plant species.
However, peanut plant is not known to synthesize mannitol.
Therefore, bacterial mtlD gene coding for mannitol 1-phosphate dehydrogenase under the control of constitutive promoter CaMV35S was introduced and overexpressed in the peanut cv.
GG 20 using Agrobacterium tumefaciens-mediated transformation.
A total of eight independent transgenic events were confirmed at molecular level by PCR, Southern blotting, and RT-PCR.
Transgenic lines had increased amount of mannitol and exhibited enhanced tolerance in response to water-deficit stress.
Improved performance of the mtlD transgenics was indicated by excised-leaf water loss assay and relative water content under water-deficit stress.
Better performance of transgenics was due to the ability of the plants to synthesize mannitol.
However, regulation of mtlD gene expression in transgenic plants remains to be elucidated.
American Psychological Association (APA)
Bhauso, Tengale Dipak& Radhakrishnan, Thankappan& Kumar, Abhay& Mishra, Gyan Prakash& Dobaria, Jentilal Ramjibhai& Patel, Kirankumar…[et al.]. 2014. Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1048377
Modern Language Association (MLA)
Bhauso, Tengale Dipak…[et al.]. Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1048377
American Medical Association (AMA)
Bhauso, Tengale Dipak& Radhakrishnan, Thankappan& Kumar, Abhay& Mishra, Gyan Prakash& Dobaria, Jentilal Ramjibhai& Patel, Kirankumar…[et al.]. Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1048377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048377