Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na +H + Antiporter Gene from the Halophyte Kosteletzkya virginica
Joint Authors
Wang, Hongyan
Tang, Xiaoli
Shao, Chuyang
Shao, Hongbo
Wang, Honglei
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A new plasma membrane Na+/H+ antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1).
The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18.
Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72–82% identity at the peptide level with other plant plasma membrane Na+/H+ antiporters.
American Psychological Association (APA)
Wang, Hongyan& Tang, Xiaoli& Shao, Chuyang& Shao, Hongbo& Wang, Honglei. 2014. Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na +H + Antiporter Gene from the Halophyte Kosteletzkya virginica. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048452
Modern Language Association (MLA)
Wang, Hongyan…[et al.]. Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na +H + Antiporter Gene from the Halophyte Kosteletzkya virginica. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1048452
American Medical Association (AMA)
Wang, Hongyan& Tang, Xiaoli& Shao, Chuyang& Shao, Hongbo& Wang, Honglei. Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na +H + Antiporter Gene from the Halophyte Kosteletzkya virginica. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048452