Noncovalent Interactions of Tiopronin-Protected Gold Nanoparticles with DNA: Two Methods to Quantify Free Energy of Binding

Abstract EN

The binding of gold nanoparticles capped with N-(2-mercaptopropionyl)glycine (Au@tiopronin) with double-stranded DNA has been investigated and quantified in terms of free energies by using two different approaches.

The first approach follows the DNA conformational changes induced by gold nanoparticles using the CD technique.

The second methodology consists in the use of pyrene-1-carboxaldehyde as a fluorescent probe.

This second procedure implies the determination of the “true” free energy of binding of the probe with DNA, after corrections through solubility measurements.

Working at different salt concentrations, the nonelectrostatic and electrostatic components of the binding free energy have been separated.

The results obtained revealed that the binding is of nonelectrostatic character, fundamentally.

The procedure used in this work could be extended to quantify the binding affinity of other AuNPs/DNA systems.